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Migraine symptoms occur, in part, because of the dilation of blood vessels in the brain. Until recently, migraine sufferers had few choices when it came to drugs to counteract this effect. But newer migraine drugs known as triptans cause constriction of blood vessels and also bring about a general interruption in the chain of chemical events that leads to a migraine. Pneumococcal 7-valent Conjugate Vaccine (Diphtheria CRM197 Protein), Prevnar , is a sterile solution of saccharides of the capsular antigens of Streptococcus pneumoniae serotypes 4, 6B, 9V, 14, 18C, 19F, and 23F individually conjugated to diphtheria CRM197 protein. Each serotype is grown in soy peptone broth. The individual polysaccharides are purified through centrifugation, precipitation, ultrafiltration, and column chromatography. The polysaccharides are chemically activated to make saccharides which are directly conjugated to the protein carrier CRM197 to form the glycoconjugate. This is effected by reductive amination. CRM197 is a nontoxic variant of diphtheria toxin isolated from cultures of Corynebacterium diphtheriae strain C7 (β197) grown in a casamino acids and yeast extract-based medium. CRM197 is purified through ultrafiltration, ammonium sulfate precipitation, and ion-exchange chromatography. The individual glycoconjugates are purified by ultrafiltration and column chromatography and are analyzed for saccharide to protein ratios, molecular size, free saccharide, and free protein.pain or burning when you urinate;CT, NJ, ME, NH

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